DNA hybridization probe for the Pseudomonas fluorescens group
نویسندگان
چکیده
منابع مشابه
DNA hybridization probe for the Pseudomonas fluorescens group.
Plasmid pHF360 was constructed from cloned rRNA genes (rDNA) of Pseudomonas aeruginosa and used as hybridization probe for the Pseudomonas fluorescens group. The probe was tested by dot and in situ colony hybridizations to chromosomal DNAs from a wide variety of organisms. pHF360 DNA hybridized exclusively to chromosomal DNAs from bacteria representing the P. fluorescens group and separated the...
متن کاملPseudomonas fluorescens
nas incognita have been previously described as being able to utilize citronellol, geraniol, and linalool (3, 6). Recently, a 50-megadalton (MDa) plasmid has been associated with the degradation of geraniol (9). In this report we describe the isolation of a Pseudomonas fluorescens s train capable of utilizing linalool as a sole carbon and energy source and demonstrate the presence of a transmis...
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In this study we attempt to determine the deactivation mechanism of a hybridization-sensitive DNA probe by using steady state absorption and fluorescence spectroscopy. Thiazole orange or 2,20Methylenebis(N-alkylbenzothiazole) is covalently linked to a single nucleotide in the DNA probe. Radiationless deactivation of the fluorophores is mainly determined by photo-induced isomerization of the met...
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Kotake and Nakayama (1) observed the conversion of kynurenine to anthranilic acid and alanine by a mammalian liver extract. Subsequently the enzyme responsible for this reaction in the mammal was partly purified (2,3) and the enzyme, prepared from vitamin Be-deficient animals, was shown to require pyridoxal phosphate for its maximal activity (2). During an investigation of tryptophan metabolism...
متن کاملSimulation-Guided DNA Probe Design for Consistently Ultraspecific Hybridization
Hybridization of complementary sequences is one of the central tenets of nucleic acid chemistry; however, the unintended binding of closely related sequences limits the accuracy of hybridization-based approaches to analysing nucleic acids. Thermodynamics-guided probe design and empirical optimization of the reaction conditions have been used to enable the discrimination of single-nucleotide var...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1986
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.52.5.1190-1194.1986